Table 2.
Stability samples result for telmisartan and amlodipine in human plasma (n=6).
| Stability test | Telmisartan | Amlodipine | ||||||
|---|---|---|---|---|---|---|---|---|
| QC (spiked concentration, ng/mL) | Mean±SD (ng/mL) | Accuracy/stability (%) | Precision (%) | QC (spiked concentration, ng/mL) | Mean±SD (ng/mL) | Accuracy/stability (%) | Precision (%) | |
| Autosampler stability (at 10 °C for 48 h) | 6.02 | 5.42±0.37 | 90.03 | 6.75 | 0.15 | 0.15±0.01 | 101.64 | 5.20 |
| 340.32 | 340.53±1.51 | 100.06 | 0.44 | 8.54 | 8.48±0.08 | 99.28 | 0.92 | |
| Wet extract stability (at 2-8 °C for 40 h) | 6.02 | 5.72 ±0.23 | 94.95 | 3.97 | 0.15 | 0.16±0.01 | 104.63 | 3.74 |
| 340.32 | 338.88±2.31 | 99.58 | 0.68 | 8.54 | 8.47±0.06 | 99.24 | 0.76 | |
| Bench top stability (10 h) | 6.02 | 5.98±0.22 | 99.31 | 3.62 | 0.15 | 0.15±0.01 | 100.58 | 3.64 |
| 340.32 | 340.12±1.85 | 99.94 | 0.54 | 8.54 | 8.51±0.08 | 99.64 | 0.92 | |
| Freeze–thaw stability | 6.02 | 6.05 ±0.11 | 100.35 | 1.78 | 0.15 | 0.14±0.01 | 95.93 | 4.20 |
| 340.32 | 340.56±1.20 | 100.07 | 0.35 | 8.54 | 8.38±0.14 | 98.14 | 1.65 | |
| Reinjection stability (24 h) | 6.02 | 6.05 ±0.13 | 100.10 | 2.18 | 0.15 | 0.15±0.01 | 101.09 | 3.55 |
| 340.32 | 340.09±1.41 | 99.86 | 0.41 | 8.54 | 8.51±0.07 | 102.00 | 0.86 | |
| Long-term stability (at −70 °C for 30 days) | 6.02 | 5.86±0.26 | 96.96 | 4.48 | 0.15 | 0.15±0.01 | 100.43 | 6.92 |
| 340.32 | 340.00±1.95 | 99.83 | 0.57 | 8.54 | 8.46±0.10 | 101.44 | 1.19 | |