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. 2017 Nov 30;10(1):63–75. doi: 10.15252/emmm.201708168

Figure 3. The mutant THPO‐conditioned culture supernatants are markedly deficient in sustaining proliferation of UT7‐TPO cells.

Figure 3

Cell proliferation measured in n = 3 independent MTT assays and expressed as absorbance units of UT7‐TPO cells incubated with medium containing 0.5 or 1.0 μl of THPO‐conditioned supernatants from HEK293T cells transfected with wild‐type (WT) or mutant (p.R119C and p.R38C) THPO‐expressing or empty vectors. Recombinant human THPO (rHuTHPO) at the concentration of 10 ng/ml was also used as control. Data are presented as means ± SD. The asterisk (*) indicates a P‐value < 0.01 with respect to WT (two‐tailed Student's t‐test). The exact P‐values are reported in Appendix Table S2. Source data are available online for this figure.