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ELISA standard curve of sparfloxacin. B and B0 are the percentages of binding in the presence and absence of sparfloxacin, respectively. The logit–log plot is obtained from ln[(B/B0)/(1−B/B0)]. Coating antigen concentration: 1 μg/mL, rabbit anti-sparfloxacin antibody dilution: 1/25600, peroxidase labeled anti-rabbit IgG dilution: 1/5000.
