Table 3.
Precision and accuracy (analysis with spiked plasma samples at three different concentrations).
| Compound name | Spiked plasma concentration (pg/mL) | Within-run (n=6) |
Between-run (n=30) |
||||
|---|---|---|---|---|---|---|---|
| Concentration measured (pg/mL, mean±SD) | Precision (CV, %) | Accuracy (%) | Concentration measured (pg/mL, mean±SD) | Precision (CV, %) | Accuracy (%) | ||
| Sibutramine | 30.0 | 29.7±0.4 | 1.3 | 99.0 | 30.5±0.5 | 1.6 | 101.7 |
| 3500.0 | 3496.4±101.4 | 2.9 | 99.9 | 3488.4±76.7 | 2.2 | 99.7 | |
| 8000.0 | 7958.5±191.0 | 2.4 | 99.5 | 7952.8±222.7 | 2.8 | 99.4 | |
| N-des methyl sibutramine | 30.0 | 29.2±0.5 | 1.6 | 97.3 | 29.8±0.4 | 1.2 | 99.3 |
| 3500.0 | 3398.5±115.5 | 3.4 | 97.1 | 3476.4±97.3 | 2.8 | 99.3 | |
| 8000.0 | 7892.6±221.0 | 2.8 | 98.7 | 7980.8±255.4 | 3.2 | 99.8 | |
| N-di desmethyl sibutramine | 30.0 | 28.9±0.5 | 1.6 | 96.3 | 29.4±0.7 | 2.4 | 98.0 |
| 3500.0 | 3426.7±95.9 | 2.8 | 97.9 | 3512.4±119.4 | 3.4 | 100.4 | |
| 8000.0 | 7896.4±189.5 | 2.4 | 98.7 | 7945.6±166.9 | 2.1 | 99.3 | |