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. 2013 Feb 8;3(4):257–269. doi: 10.1016/j.jpha.2013.01.004

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© 2013 Xi’an Jiaotong University. Production and hosting by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).

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Fig. 10 — FT-IR spectra in the region of 1800–900 cm⁻¹ for free BSA (0.10 mM), the BSA–indigotin complex (the molar ratio of BSA/indigotin was maintained at 1:3), and its corresponding difference spectra (indicated in the figure). The contribution of indigotin was subtracted from different spectra in this region.