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Effects of the site probe on the binding constants of indigotin to BSA/HSA.
| System | Without |
With IB |
With PB |
With DIG |
||||
|---|---|---|---|---|---|---|---|---|
| K (×10−5 L/mol) | R | K (×10−5 L/mol) | R | K (×10−5 L/mol) | R | K(×10−5 L/mol) | R | |
| BSA–indigotin | 2.186 | 0.9978 | 3.318 | 0.9994 | 1.585 | 0.9991 | 2.915 | 0.9996 |
| HSA–indigotin | 6.005 | 0.9991 | 6.167 | 0.9990 | 1.922 | 0.9979 | 4.858 | 0.9989 |
