Antibodies |
Anti-Myc |
Santa Cruz |
Cat. sc-40; RRID: AB_627268 |
Anti-Hsp60 |
Abcam |
Cat. ab46798; RRID: AB_881444 |
Anti -CD68 |
Abcam |
Cat. ab955; RRID: AB_307338 |
Anti -Ly6g |
Abcam |
Cat. ab25377; RRID: AB_470492 |
Anti-RIP1 |
BD Biosciences |
Cat. 551042; RRID: AB_394015 |
Anti-Nur77 |
BD Biosciences |
Cat. 554088; RRID: AB_395232 |
Anti-Ubiquitin |
Cell Signal Technology |
Cat. 3933S; RRID: AB_394015 |
Anti-β-actin |
Cell Signal Technology |
Cat. 4970S; RRID: AB_2223172 |
Anti-IκBα |
Cell Signal Technology |
Cat. 4814S; RRID: AB_10693636 |
Anti-LC3 |
Cell Signal Technology |
Cat. 4108S; RRID: AB_2137703 |
Anti-p-Ikkα/Ikkβ |
Cell Signal Technology |
Cat. 2078s; RRID: AB_2079379 |
Anti-Parp |
Cell Signal Technology |
Cat. 9542; RRID: AB_2160739 |
Anti-TNFR1 |
Santa Cruz Biotechnology |
Cat. sc-8436; RRID: AB_628377 |
Anti-Nur77 (M-210) |
Santa Cruz Biotechnology |
Cat. sc-5569; RRID: AB_653373 |
Anti-LC3B |
Santa Cruz Biotechnology |
Cat. sc-28266; RRID: AB_2137719 |
Anti-p65 |
Santa Cruz Biotechnology |
Cat. sc-109; RRID: AB_632039 |
Anti-GST |
Santa Cruz Biotechnology |
Cat. sc-138; RRID: AB_627677 |
Anti-TRAF2 |
Santa Cruz Biotechnology |
Cat. sc-7187; RRID: AB_793340 |
Anti-IκBα |
Santa Cruz Biotechnology |
Cat. sc-371; RRID: AB_793340 |
Anti -FLAG |
Sigma |
Cat. F1804; RRID: AB_262044 |
Goat Anti-Mouse IgG F(ab′)2 Secondary Antibody, HRP conjugate |
Pierce |
Cat. 31436; RRID: BDSC_31436 |
Goat anti-Rabbit IgG F(ab′)2 Secondary Antibody, HRP conjugate |
Pierce |
Cat. 31461; RRID: BDSC_31461 |
Chemicals, Peptides, and Recombinant Proteins |
Proteinase K |
Amresco |
Cat. 0706 |
Wortmannin |
Beyotime |
Cat. S1952 |
mouse IL-1β ELISA kit |
Elabscience Biotechnology |
Cat. E-EL-M0037c |
mouse IL-6 ELISA kit |
Elabscience Biotechnology |
Cat. E-EL-M0044c |
Collagenase Type IV |
GIBCO |
Cat. 17104-019 |
G418 Sulfate |
Merck |
Cat.345810-5GM |
Recombinant Human TNFa protein |
Peprotech |
Cat. 300-01a-50 |
Lipopolysaccharide |
Sigma |
Cat. L2630 |
D-Galactosamine |
Sigma |
Cat. G0500 |
Chloroquine |
Sigma |
Cat. C6628-25G |
Dexamethasone |
Sigma |
Cat. D8040 |
MG132 |
Sigma |
Cat. C2211-5MG |
9-cis-RA |
Sigma |
Cat. R4643-1MG |
Nur77 siRNA |
Sigma |
Cat.SASI_Hs02_0 0333289 |
TRAF2 siRNA |
Sigma |
Cat.SASI_Hs01_0 0095954 |
RXRα siRNA |
Sigma |
Cat.SASI_HS01_0 0097639 |
Control siRNA |
Sigma |
Cat.sc-37007 |
CD3254 |
Santa Cruz Biotechnology |
Cat. sc-358786 |
Critical Commercial Assays |
PageRuler Prest Protein Ladder |
Fermantas |
Cat. 26617 |
Protein G Agarose, Fast Flow |
Millipore |
Cat.16-266 |
Aspartate aminotransferase Assay Kit Nanjing Jiancheng |
Bioengineering Institute |
Cat. C010-1 |
Alanine aminotransferase Assay Kit Nanjing Jiancheng |
Bioengineering Institute |
Cat. C009-1 |
FastStart Universal SYBR Green Master(ROX) |
Roche |
Cat. 4913850001 |
TianScript RT kit |
TianGen Biotechnology |
Cat. KR104-02 |
Pierce BCA Protein Assay Kit |
Thermo Scientific |
Cat. 23225 |
Experimental Models: Cell Lines |
Mouse: primary embryonic fibroblasts |
This paper |
C57BL/6J |
HepG2 |
ATCC |
HB-8065 |
HEK293T |
ATCC |
CRL-3216 |
LO2 |
Cell Bank, Shanghai Cell Biology Institute |
GNHu 6 |
SMMC-7721 |
Cell Bank, Shanghai Cell Biology Institute |
TCHu 52 |
QGY-7703 |
Cell Bank, Shanghai Cell Biology Institute |
TCHu 43 |
HeLa |
ATCC |
CCL2 |
H460 |
ATCC |
HTB-177 |
Experimental Models: Organisms/Strains |
|
|
Mouse: C57BL/6J |
The Jackson Laboratory |
Stock No: 000664 |
Mouse:B6;129S2-Nr4a1tm1Jmi/J |
The Jackson Laboratory |
Stock No: 006187 |
pcmv-myc-Nur77 |
This study |
Kolluri et al., 2008 |
pcmv-myc-Nur77/L506W |
This study |
N/A |
pcmv-myc-Nur77/L555W |
This study |
N/A |
pcmv-myc-Nur77/L556W |
This study |
N/A |
pcmv-myc-Nur77ΔAF2 |
This study |
N/A |
pcmv-myc-Nur77ΔAB |
This study |
Li et al., 2000 |
pcmv-myc-Nur77ΔDBD |
This study |
Li et al., 2000 |
pcmv-myc-Nur77ΔAB/ΔDBD |
This study |
Kolluri et al., 2008 |
pcmvmyc-Nur77ΔLBD |
This study |
Lin et al., 2004 |
pEGFP-C1-Nur77 |
This study |
Li et al., 2000 |
pET-15b-Nur77/LBD |
This study |
N/A |
pFlag-cmv-2-TRAF2 |
This study |
Zeng et al., 2015 |
pFlag-cmv-2-TRAF2(1-249) |
This study |
N/A |
pFlag-cmv-2-TRAF2(97-356) |
This study |
N/A |
pFlag-cmv-2-TRAF2(272-501) |
This study |
N/A |
GST-TRAF2 |
This study |
Zeng et al., 2015 |
GFP-LC3 |
This study |
N/A |
RFP-LC3 |
This study |
N/A |
HA-Ub |
This study |
N/A |
HA-UbK63R |
This study |
N/A |
pBind-Nur77 |
This study |
N/A |
pBind-GR/LBD |
This study |
Zeng et al., 2015 |
pG5 |
This study |
Zeng et al., 2015 |
pFlag-cmv-2-p62 |
This study |
N/A |
pFlag-cmv-2-p62-ΔUBA |
This study |
N/A |
Sequence-Based Reagents |
IL-6 forward: CCAGAGATACAAAGAAATGATGG |
This study |
N/A |
IL-6 reverse: ACTCCAGAAGACCAGAGGAAAT |
This study |
N/A |
IL-1β forward: TGGTGTGTGACGTTCCCATT |
This study |
N/A |
IL-1β reverse: CAGCACGAGGCTTTTTTGTTG |
This study |
N/A |
Software and Algorithms |
LSM-510 confocal laser scanning microscope system (CarlZeiss) |
open source |
https://www.zeiss.com/global/home.html |
image analysis system (Olympus, Tokyo, Japan) |
open source |
http://www.olympus-ims.com/en/microscope/software/ |
GraphPad Prism |
open source |
http://www.graphpad.com/ |