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. Author manuscript; available in PMC: 2018 Jan 10.
Published in final edited form as: Mol Cell. 2017 Apr 6;66(1):141–153.e6. doi: 10.1016/j.molcel.2017.03.008
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Anti-Myc Santa Cruz Cat. sc-40; RRID: AB_627268
Anti-Hsp60 Abcam Cat. ab46798; RRID: AB_881444
Anti -CD68 Abcam Cat. ab955; RRID: AB_307338
Anti -Ly6g Abcam Cat. ab25377; RRID: AB_470492
Anti-RIP1 BD Biosciences Cat. 551042; RRID: AB_394015
Anti-Nur77 BD Biosciences Cat. 554088; RRID: AB_395232
Anti-Ubiquitin Cell Signal Technology Cat. 3933S; RRID: AB_394015
Anti-β-actin Cell Signal Technology Cat. 4970S; RRID: AB_2223172
Anti-IκBα Cell Signal Technology Cat. 4814S; RRID: AB_10693636
Anti-LC3 Cell Signal Technology Cat. 4108S; RRID: AB_2137703
Anti-p-Ikkα/Ikkβ Cell Signal Technology Cat. 2078s; RRID: AB_2079379
Anti-Parp Cell Signal Technology Cat. 9542; RRID: AB_2160739
Anti-TNFR1 Santa Cruz Biotechnology Cat. sc-8436; RRID: AB_628377
Anti-Nur77 (M-210) Santa Cruz Biotechnology Cat. sc-5569; RRID: AB_653373
Anti-LC3B Santa Cruz Biotechnology Cat. sc-28266; RRID: AB_2137719
Anti-p65 Santa Cruz Biotechnology Cat. sc-109; RRID: AB_632039
Anti-GST Santa Cruz Biotechnology Cat. sc-138; RRID: AB_627677
Anti-TRAF2 Santa Cruz Biotechnology Cat. sc-7187; RRID: AB_793340
Anti-IκBα Santa Cruz Biotechnology Cat. sc-371; RRID: AB_793340
Anti -FLAG Sigma Cat. F1804; RRID: AB_262044
Goat Anti-Mouse IgG F(ab′)2 Secondary Antibody, HRP conjugate Pierce Cat. 31436; RRID: BDSC_31436
Goat anti-Rabbit IgG F(ab′)2 Secondary Antibody, HRP conjugate Pierce Cat. 31461; RRID: BDSC_31461
Chemicals, Peptides, and Recombinant Proteins
Proteinase K Amresco Cat. 0706
Wortmannin Beyotime Cat. S1952
mouse IL-1β ELISA kit Elabscience Biotechnology Cat. E-EL-M0037c
mouse IL-6 ELISA kit Elabscience Biotechnology Cat. E-EL-M0044c
Collagenase Type IV GIBCO Cat. 17104-019
G418 Sulfate Merck Cat.345810-5GM
Recombinant Human TNFa protein Peprotech Cat. 300-01a-50
Lipopolysaccharide Sigma Cat. L2630
D-Galactosamine Sigma Cat. G0500
Chloroquine Sigma Cat. C6628-25G
Dexamethasone Sigma Cat. D8040
MG132 Sigma Cat. C2211-5MG
9-cis-RA Sigma Cat. R4643-1MG
Nur77 siRNA Sigma Cat.SASI_Hs02_0 0333289
TRAF2 siRNA Sigma Cat.SASI_Hs01_0 0095954
RXRα siRNA Sigma Cat.SASI_HS01_0 0097639
Control siRNA Sigma Cat.sc-37007
CD3254 Santa Cruz Biotechnology Cat. sc-358786
Critical Commercial Assays
PageRuler Prest Protein Ladder Fermantas Cat. 26617
Protein G Agarose, Fast Flow Millipore Cat.16-266
Aspartate aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat. C010-1
Alanine aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat. C009-1
FastStart Universal SYBR Green Master(ROX) Roche Cat. 4913850001
TianScript RT kit TianGen Biotechnology Cat. KR104-02
Pierce BCA Protein Assay Kit Thermo Scientific Cat. 23225
Experimental Models: Cell Lines
Mouse: primary embryonic fibroblasts This paper C57BL/6J
HepG2 ATCC HB-8065
HEK293T ATCC CRL-3216
LO2 Cell Bank, Shanghai Cell Biology Institute GNHu 6
SMMC-7721 Cell Bank, Shanghai Cell Biology Institute TCHu 52
QGY-7703 Cell Bank, Shanghai Cell Biology Institute TCHu 43
HeLa ATCC CCL2
H460 ATCC HTB-177
Experimental Models: Organisms/Strains
Mouse: C57BL/6J The Jackson Laboratory Stock No: 000664
Mouse:B6;129S2-Nr4a1tm1Jmi/J The Jackson Laboratory Stock No: 006187
pcmv-myc-Nur77 This study Kolluri et al., 2008
pcmv-myc-Nur77/L506W This study N/A
pcmv-myc-Nur77/L555W This study N/A
pcmv-myc-Nur77/L556W This study N/A
pcmv-myc-Nur77ΔAF2 This study N/A
pcmv-myc-Nur77ΔAB This study Li et al., 2000
pcmv-myc-Nur77ΔDBD This study Li et al., 2000
pcmv-myc-Nur77ΔAB/ΔDBD This study Kolluri et al., 2008
pcmvmyc-Nur77ΔLBD This study Lin et al., 2004
pEGFP-C1-Nur77 This study Li et al., 2000
pET-15b-Nur77/LBD This study N/A
pFlag-cmv-2-TRAF2 This study Zeng et al., 2015
pFlag-cmv-2-TRAF2(1-249) This study N/A
pFlag-cmv-2-TRAF2(97-356) This study N/A
pFlag-cmv-2-TRAF2(272-501) This study N/A
GST-TRAF2 This study Zeng et al., 2015
GFP-LC3 This study N/A
RFP-LC3 This study N/A
HA-Ub This study N/A
HA-UbK63R This study N/A
pBind-Nur77 This study N/A
pBind-GR/LBD This study Zeng et al., 2015
pG5 This study Zeng et al., 2015
pFlag-cmv-2-p62 This study N/A
pFlag-cmv-2-p62-ΔUBA This study N/A
Sequence-Based Reagents
IL-6 forward: CCAGAGATACAAAGAAATGATGG This study N/A
IL-6 reverse: ACTCCAGAAGACCAGAGGAAAT This study N/A
IL-1β forward: TGGTGTGTGACGTTCCCATT This study N/A
IL-1β reverse: CAGCACGAGGCTTTTTTGTTG This study N/A
Software and Algorithms
LSM-510 confocal laser scanning microscope system (CarlZeiss) open source https://www.zeiss.com/global/home.html
image analysis system (Olympus, Tokyo, Japan) open source http://www.olympus-ims.com/en/microscope/software/
GraphPad Prism open source http://www.graphpad.com/
HHS Vulnerability Disclosure