Table 1.
Analytical results obtained for determining AMK base content in AMK sulfate injections by the proposed CFS-FTIR-DS method.
| Band peak parameters (units in cm−1) of the first derivative spectra |
Parameters derived from linear regressiona: Y=a+bX |
||||||
|---|---|---|---|---|---|---|---|
| Measurement mode | Location | Baseline | a±SD (×10−3) | b±SD (×10−3) | r | LOD | RSD |
| Total height | 1514 | Zero abs | 0.08±0.02 | 0.0648±0.0004 | 0.9996 | 2.1 | 0.004 |
| Corrected height | 1514 | 1538–1469 | 0.05±0.07 | 0.0728±0.0007 | 0.9995 | 2.2 | 0.005 |
| Total area | 1514 | Zero abs | 1±1 | 2.25±0.02 | 0.9987 | 3.5 | 0.30 |
| Corrected area | 1514 | 1538–1469 | 1.0±0.6 | 2.81±0.01 | 0.9997 | 1.6 | 0.15 |
a
Calibration curves obtained using eight standards of AMK sulfate, in triplicate, registered as its equivalent AMK concentration ranging from 7.7 to 77.0 (mg/mL); where X is the explanatory variable and Y is the dependent variable; the slope of the line is b, and a is the Y-intercept; SD=standard deviation; r=correlation coefficient; LOD=limit of detection in mg/mL AMK base calculated as 3σ(1/slope); RSD=relative standard deviation (%).