Table 1.
Modifications to PT subunits. Residues 58 and 62 of S5 were substituted singly and in combination, in order to provide steric hindrance to the interaction between PT and ATP. PTS1GS, PTS4GS, PTS1GSDM and PTS4GSDM were engineered with peptide sequences that are targets for tyrosine sulfation and N-glycosylation (Plaut and Carbonetti 2008) in addition to the indicated changes to residues of S5.
| Name | Residues changed | Source |
|---|---|---|
| PTSM58 | S5: G58N | This study |
| PTSM62 | S5: S62E | This study |
| PTDM | S5: G58N and S62E | This study |
| PTS1GS | S1: tyrosine sulfation and | Plaut and |
| N-glycosylation sites added | Carbonetti (2008) | |
| PTS4GS | S4: tyrosine sulfation and | Plaut and |
| N-glycosylation sites added | Carbonetti (2008) | |
| PTS1GSDM | S1: tyrosine sulfation and | This study |
| N-glycosylation sites added | ||
| S5: G58N and S62E | ||
| PTS4GSDM | S4: tyrosine sulfation and | This study |
| N-glycosylation sites added | ||
| S5: G58N and S62E |