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. Author manuscript; available in PMC: 2018 Jan 10.
Published in final edited form as: ACS Chem Biol. 2017 Jan 24;12(3):724–734. doi: 10.1021/acschembio.6b00782

Figure 5.

Figure 5

EG1 treatment leads to gene expression changes consistent with reduction in Pax2 activity in an ex vivo organ culture model. (A) Whole mount in situ hybridization for Cited1 shows that EG1 inhibits expression of a Pax2 target gene expressed in renal epithelial progenitor cells (arrows). Note a loss of strong Cited1 expression in EG1 treated cultures at the ureteric bud tips. (B) Quantitative RT-PCR for mRNAs of critical regulators of nephrogenesis shows reduced expression of these genes following EG1 treatment. (C) Quantitative RT-PCR for interstitial stroma and paraxial mesodermal mRNAs show increased expression following EG1 treatment.