Figure 5.

Increased pCaMKIIα immunoreactivity in the spinal dorsal horn of EAE mice was reduced by KN93 and CaMKIIα siRNA. A, pCaMKIIα immunoreactivity in the spinal dorsal horn were observed using immunofluorescence. B, Immunoreactivity of pCaMKIIα was significantly increased in the spinal dorsal horn of EAE mice. Prophylactic KN93 administration or CaMKIIα siRNA significantly reduced the pCaMKIIα immunoreactivity. Data are expressed as the mean ± SEM. ***p < 0.001 compared with the sham immune group; #p < 0.05, ##p < 0.01 compared with the saline-treated EAE group. Scale bar, 100 μm.