Skip to main content
. 2017 Jul 7;20(1):78–91. doi: 10.1093/neuonc/nox128

Fig. 1.

Fig. 1

WEE1 is activated by PI3K inhibition and regulates PI3K inhibition–induced G2/M arrest. (A) GSC25, GSC240, GSC23, and U251 cells were treated with BKM at 1 μM for 72 hours. Cell cycle distribution was analyzed by flow cytometry. Statistical analysis showed significant difference of G2/M distribution between control and BKM treatment. (B) Activity of pAkt, pWEE1, and pCdc2 was detected by specific phosphorylation antibodies after BKM treatment. (C) GSC23 cells were treated with vehicle control, BKM alone (1 μM), MK1775 alone (0.5 μM), or combination for 24 hours, and pCdc2 and pAkt were detected by western blot. (D) GSC23 cells were treated with vehicle control, BKM alone (1 μM), MK1775 alone (0.5 μM), or combination for 72 hours, and cell cycle distribution was analyzed by flow cytometry. P-value for G2 phase cell fraction.