Fig. 2.

Combination of BKM and MK1775 suppressed GBM cell proliferation. (A) GSC23 cells were treated with BKM, MK1775, or combination for 72 hours. Neurosphere formation was observed by microscope. (B) GSC23 cells were treated with 1 μM BKM, 0.5 μM MK1775, or combination. Cell numbers were counted every day and a cell proliferation curve was plotted. P < 0.0001 for combination vs control and BKM; P = 0.0008 for combination vs MK1775. (C–D) U251 cells were treated with BKM, MK1775, or combination for 72 hours, cell morphology was observed under microscope (C) and cell number was counted (D). (E) U251 cells in chamber slides were stained with Ki-67. Representative immunofluorescent images of Ki-67 staining were shown and a bar figure was presented with mean + SD. Scale bars, 100 microns for (A) and (C), 50 microns for (E).