Figure 6.
Food intake, blood glucose, and c-Fos expression in VLM after CNO injection in C1m+C1rAAV-hM3D rats. (A) Food intake and (B) blood glucose levels during the 4-hour (for feeding) or 6-hour (for glucose) period after control (intraperitoneal saline), 2DG (250 mg/kg subcutaneously), or CNO (1 mg/kg intraperitoneally) administration in female Th-Cre+ rats injected unilaterally with AAV-hM3D into both C1m and C1r (n = 8 rats per treatment). *P < 0.001 vs saline control at the same time point (by post hoc Student-Newman-Keuls test after two-way repeated-measures analysis of variance). (C) Representative double staining of c-Fos (green) and mCherry (red) in female C1m+C1rAAV-hM3D Th-Cre+ rats 2 hours after saline (control) or CNO (1 mg/kg intraperitoneal) injection. Scale bar = 25 µm. (D) Cell numbers of c-Fos/mCherry in C1m+C1r averaged from three to four rats (seven sections per rat). *P < 0.001 vs saline-injected control (by unpaired t test). (E) Distribution of mCherry expression in VLM, expressed as percentage of rats with ≥5 mCherry-positive cells per site at each bregma level in C1m+C1rAAV-hM3D Th-Cre+ rats. Distance caudal to bregma is shown on x-axis.