Appendix.
Antibody Table
| Peptide/Protein Target | Antigen Sequence (if Known) | Name of Antibody | Manufacturer, Catalog No., and/or Name of Individual Providing the Antibody | Species Raised in; Monoclonal or Polyclonal | Dilution Used | RRID |
|---|---|---|---|---|---|---|
| ACTH | Human ACTH AA 1-24 | Polyclonal rabbit anti-human ACTH | DakoCytomation, A0571 | Rabbit; polyclonal | 1:250 | No longer commercially available |
| Mouse β-actin | NA | β-Actin (D6A8) rabbit mAb | Cell Signaling Technology, 8457 | Rabbit; monoclonal | 1:1000 | AB_10950489 |
| Chicken α-tubulin | NA | Mouse anti–α-tubulin monoclonal antibody, unconjugated, clone DM1A | Sigma-Aldrich, T9026 | Mouse; monoclonal | 1:5000 | AB_477593 |
| Rat PIT1 | NA | Anti-PIT1 | Simon Rhodes | Rabbit; polyclonal | 1:1000 | Not commercially available |
| Rat βLH | NA | Anti-rβLH-IC | National Hormone and Peptide Program, Dr. A.F. Parlow | Rabbit; polyclonal | 1:1000 | AB_2665533 |
| Human phosphorylated histone H3 | Histone H3 at serine 10 | Anti-phosphorylated histone H3 (Ser10) antibody | Millipore | Rabbit; polyclonal | 1:1000 | AB_310177 |
Abbreviations: AA, amino acid; AB, antibody; LHβ, luteinizing hormone-β; mAb, monoclonal antibody; NA, not available; rβLH-IC, rat LH β for immunocytochemistry; RRID, Research Resource Identifier.