Table 1.
IpaD Mutant | Kd (μM) ± SDa |
---|---|
Wild-type IpaD | 9.3 ± 1.8 |
D144A | 10.5 ± 2.5 |
N146A | 10.9 ± 0.2 |
Y149A | 10.8 ± 0.3 |
D144A/N146A | 9.7 ± 1.7 |
D144A/Y149A | 10.7 ± 1.5 |
N146A/Y149A | 9.9 ± 2.3 |
D144A/N146A/Y149A | 8.3 ± 2.2 |
Apparent Kd values between IpaD and fluorescein-DOC reported as the mean ± standard deviation resulting from three independent analyses. There is no statistical difference among the means (one-way ANOVA (F(7,16) = 0.803, p = 0.597)).