KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-mouse CD3 (clone: 145-2C11) | ThermoFisher Scientific | 47-0031-82 |
| Anti-mouse CD4 (GK1.5) | Biolegend | 100406 |
| Anti-mouse CD8 (53-6.7) | Biolegend | 100706 |
| Anti-mouse/human CD11b (M1/70) | Biolegend | 101212 |
| Anti-mouse CD11c (N418) | Biolegend | 117317 |
| Anti-mouse CD19 (1D3) | ThermoFisher Scientific | 47-0193-82 |
| Anti-mouse CD25 (3C7) | Biolegend | 101915 |
| Anti-mouse/rat/human CD27 (LG.3A10) | Biolegend | 124216 |
| Anti-mouse CD28 (37.51) | ||
| Anti-mouse CD45.1 (A20) | Biolegend | 48-0453-82 |
| Anti-mouse CD45.2 (104) | Biolegend | 109822 |
| Anti-mouse CD49a (HMα1) | Biolegend | 142604 |
| Anti-mouse CD49b (DX5) | Biolegend | 108910 |
| Anti-mouse CD90.2 (30-H12) | Biolegend | 105324 |
| Anti-mouse CD107a (1D4B) | Biolegend | 121612 |
| Anti-mouse/pig CD117 (2B8) | ThermoFisher Scientific | 47-1171-80 |
| Anti-mouse CD122 (TM-β1) | Biolegend | 123213 |
| Anti-mouse CD127 (A7R34) | Biolegend | 135010 |
| Anti-mouse 2B4 (m2B4(B6)458.1) | Biolegend | 133508 |
| Anti-mouse α4β7 Integrin (DATK32) | Biolegend | 120612 |
| Anti-BrdU (Bu20a) | Biolegend | 339812 |
| Anti-mouse Eomes (Dan11mag) | ThermoFisher Scientific | 50-4875-82 |
| Anti-mouse F4/80 (BM8) | Biolegend | 123113 |
| Anti-mouse Flt3 (A2F10) | Biolegend | 135314 |
| Anti-mouse GATA3 (L50-823) | BD Biosciences | 135314 |
| Anti-mouse IFN-γ (XMG1.2) | Biolegend | 505826 |
| Anti-mouse IL-2 (S4B6) | BioXcell | BE0043-1-5MG |
| Anti-mouse IL-4 (11B11) | Biolegend | 504107 |
| Anti-mouse IL-5 (TRFK5) | ThermoFisher Scientific | 12-7052-81 |
| Anti-mouse IL-13 (eBio13A) | ThermoFisher Scientific | 53-7133-80 |
| Anti-mouse IL-22 (IL22JOP) | ThermoFisher Scientific | 17-7222-82 |
| Anti-mouse Ly6G (1A8) | Biolegend | 127606 |
| Anti-mouse MHC-II (M5/114.15.2) | Biolegend | 107621 |
| Anti-mouse NK1.1 (PK136) | Biolegend | 108745 |
| Anti-mouse NKp46 (29A1.4) | Biolegend | 137606 |
| Anti-mouse Rorγt (B2D) | ThermoFisher | 12-6981-82 |
| Scientific | ||
| Anti-mouse Sca-1 (D7) | Biolegend | 108131 |
| Anti-mouse Siglec-F (E50-2440) | BD Biosciences | 552126 |
| Anti-mouse p-STAT4 (38/pSTAT4) | BD Biosciences | 558137 |
| Anti-mouse/human STAT5A/B (Rabbit polyclonal IgG) | R&D Systems | AF2168 |
| Anti-mouse p-STAT5 (47/Stat5(pY694)) | BD Biosciences | 612567 |
| Anti-mouse T1/ST2 (Biotin; DJ8) | MD Bioproducts | 101001B |
| Anti-mouse/human T-bet (4B10) | Biolegend | 644824 |
| Anti-mouse TCRβ (H57-597) | Biolegend | 109212 |
| Anti-mouse Ter119 (TER-119) | Biolegend | 116206 |
| Anti-H3K27ac (Rabbit polyclonal) | Abcam | ab4729 |
| Anti-H3K36me3 (Rabbit polyclonal) | Abcam | ab9050 |
| Streptavidin | Biolegend | 405207 |
| 7-AAD | Sigma | A9400-1MG |
| DAPI | Sigma | D9542-5MG |
| Live/Dead Aqua | ThermoFisher Scientific | L34966 |
| Bacterial and Virus Strains | ||
| Listeria monocytogenes 1043S | Laboratory of E. John Wherry | N/A |
| Samonella enterica serovar Typhimurium (SL1344) | Laboratory of Igor Brodsky | N/A |
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| BrdU | Sigma | B5002-1G |
| Papain | Millipore | 5125-50GM |
| Liberase TM | Sigma | 5401127001 |
| Collagenase D | Sigma | 11088866001 |
| Collagenase 4 | Worthington Biochemical | LS004188 |
| DNase I | Sigma | DN25-1G |
| Percoll | GE Healthcare | 17-0891-01 |
| Recombinant human IL-2 | Peprotech | 200-02 |
| Recombinant mouse IL-2 | Peprotech | 212-12 |
| Recombinant mouse IL-3 | Biolegend | 575502 |
| Recombinant mouse IL-6 | Biolegend | 575702 |
| Recombinant mouse IL-12 | Biolegend | 577002 |
| Recombinant mouse IL-15/IL-15Rα | ThermoFisher Scientific | 14-8152-80 |
| Recombinant mouse IL-18 | MBL International | B002-5 |
| Recombinant mouse IL-23 | ThermoFisher Scientific | 14-8231-63 |
| Recombinant mouse IL-33 | Biolegend | 580504 |
| Recombinant mouse SCF | Biolegend | 579702 |
| Critical Commercial Assays | ||
| KAPA SYRBR Fast | KAPA Biosystems | KK4618 |
| Cells-to-CT 1-Step TaqMan Kit | ThermoFisher Scientific | A25605 |
| Protein G Dynabeads | ThermoFisher Scientific | 10004D |
| BioMag Goat Anti-Rat IgG | Qiagen | 310107 |
| Foxp3 Transcription Factor Staining Kit | ThermoFisher Scientific | 00-5523-00 |
| Cytofix/Cytoperm | BD Biosciences | 554722 |
| Permeabilization buffer | ThermoFisher Scientific | 00-8333-56 |
| BrdU staining buffer set | ThermoFisher Scientific | 00-5525-00 |
| Deposited Data | ||
| Raw RNA-seq and ATAC-seq data | This paper | GEO: GSE101459 |
| ILC RNA-seq and ATAC-seq tracks | (Shih et al., 2016) | GEO: GSE77695 |
| RNA-seq from Id2-deficient NK cells | (Delconte et al., 2016) | GEO: GSE76466 |
| STAT5 ChIP-seq tracks from NK cells | (Villarino et al., 2017, In Press) | GEO: GSE100674 |
| Experimental Models: Cell Lines | ||
| RMA | Laboratory of Taku Kambayashi | N/A |
| RMA-S | Laboratory of Taku Kambayashi | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: Rroid−/− | This paper | N/A |
| Mouse: Rroid-P1−/− | This paper | N/A |
| Mouse: Rroid-E1−/− | This paper | N/A |
| Mouse: C57BL/6 | Jackson Laboratory | JAX: 00664 |
| Mouse: B6.SJL-Ptprca Pepcb/Boy (CD45.1+) | Jackson Laboratory | JAX: 002014 |
| Mouse: Zbtb16tm1.1(EGFP/cre)Aben (PLZFGFPcre) | Jackson Laboratory | JAX: 024529 |
| Mouse: Stat5a−/− Stat5b+/− | Laboratory of John J. O’Shea | N/A |
| Mouse: Tbx21−/− | Laboratory of Christopher Hunter | N/A |
| Oligonucleotides | ||
| Primers for PCR and qPCR located in Table S2 | ||
|
Rroid-5′ sgRNA:
GATTCACAGGCACTTGTTTCG TTTTAGAGCTAGAAATAGCAAGTTAAA ATAAGGCTAGTCCGTTATCAACTTGA AAAAGTGGCACCGAGTCGGTGCTTTTTT |
This paper | N/A |
|
Rroid-3′ sgRNA:
GCTATAGGTGTTAATTCTGTG TTTTAGAGCTAGAAATAGCAAGTTAAA ATAAGGCTAGTCCGTTATCAACTTGA AAAAGTGGCACCGAGTCGGTGCTTTTTT |
This paper | N/A |
|
Rroid-P1-5′ sgRNA:
GTATGATAAACTCACAATGG GTTTTAGAGCTAGAAATAGCAAGTTAAAA TAAGGCTAGTCCGTTATCAACTTGA AAAAGTGGCACCGAGTCGGTGCTTTTTT |
This paper | N/A |
| Rroid-P1-3′ sgRNA: | This paper | N/A |
| TCGCTTCAGAGGGCACTATAGTTTTA GAGCTAGAAA |
||
| TAGCAAGTTAAAATAAGGCTAGTCCG TTATCAACTT |
||
| GAAAAAGTGGCACCGAGTCGGTGCTTTTTT | ||
|
Rroid-RE1-5′ sgRNA:
TGTCCAAGGATGTCCATATT GTTTTAGAGCTAGAAATAGCAAGTTAAAAT AAGGCTAGTCCGTTATCAACTTGAA AAAGTGGCACCGAGTCGGTGCTTTTTT |
This paper | N/A |
|
Rroid-RE1-3′ sgRNA:
CATCCGAAGATCCCTGTATG GTTTTAGAGCTAGAAATAGCAAGTTAAAA TAAGGCTAGTCCGTTATCAACTTGAA AAAGTGGCACCGAGTCGGTGCTTTTTT |
This paper | N/A |
| Recombinant DNA | ||
| RP23-300A18 | Bacpac | N/A |
| RP23-342B16 | Bacpac | N/A |
| Software and Algorithms | ||
| FlowJo v10.3 | Tree Star | https://www.flowjo.com/solutions/flowjo |
| Graphpad Prism 7 | GraphPad Software | https://www.graphpad.com/ |
| Fiji | (Schindelin et al., 2012) | https://fiji.sc/ |
| Other | ||