Figure 7.

Overexpression of RGL1 partially rescues the early flowering phenotype of WRKY75 overexpressing plants. A, The flowering phenotypes of the indicated genotypes. Plants were grown under long days at 22°C. Three independent experiments were performed with each replica containing more than 30 plants for each line. Representative plants were photographed. B, Expression of WRKY75 and RGL1 in the indicated genotypes. RNA was isolated form 3-week-old leaves of the indicated genotype. ACTIN2 gene was used as an internal control. Values are mean ± sd of three independent biological replicates. C and D, Flowering phenotype of the indicated genotypes assessed by RLN (D) and DTF (E) under LD conditions. Values are mean ± sd of approximately 30 plants (**P < 0.01). Asterisks indicate Student’s t-test significant differences. The experiments were repeated at least three times with similar results. E, Expression of FT in the indicated genotypes. Ten-d-old plants grown under normal growth conditions (22°C, LD) were harvested at ZT15 for total RNA extraction and qRT-PCR assays. Transcript levels of FT in untreated Col-0 leaves were arbitrarily set to 1. Values are mean ± sd of three independent biological replicates. Asterisks indicate Student’s t-test significant differences (**P < 0.01).