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. 2017 Nov 13;176(1):552–565. doi: 10.1104/pp.17.00755

Table I. Results of inhibition assays of cell death in rice protoplasts and N. benthamiana leaves.

ND, Not determined.

Gene Treatment
LaCl3a
Darkb
Bcl-xlc
LUC Activity in RPd Cell Death in NBLe LUC Activity in RP Cell Death in NBL LUC Activity in RP Cell Death in NBL
GFP 1.00 ± 0.19 0/8 1.00 ± 0.34 0/8 ND 0/8
NlMLP 0.74 ± 0.17 1/8 0.14 ± 0.02* 8/8 ND 0/8
BAX 0.66 ± 0.21 3/8 0.09 ± 0.02* 7/8 ND 0/8
INF1 0.92 ± 0.34 3/8 0.38 ± 0.11* 7/8 ND 6/8
a

LaCl3 was applied to protoplasts immediately after transfection at a final concentration of 1 mm. For agroinfiltration of N. benthamiana leaves, LaCl3 was added to resuspended A. tumefaciens cultures at a final concentration of 1 mm.

b

Rice protoplasts and N. benthamiana leaves were incubated in the dark for 30 min before transfection and maintained in the dark after transfection.

c

N. benthamiana leaves were preinfiltrated with A. tumefaciens cells harboring the Bcl-xl expression vector.

d

LUC activity in rice protoplasts. Data represent means ± se of three repeats. Asterisks indicate significant differences compared with the corresponding GFP (*, P < 0.05, Student’s t test).

e

Number of cell death sites/total number of infiltrated leaves of N. benthamiana.