Table I. Results of inhibition assays of cell death in rice protoplasts and N. benthamiana leaves.
ND, Not determined.
Gene | Treatment |
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LaCl3a |
Darkb |
Bcl-xlc |
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LUC Activity in RPd | Cell Death in NBLe | LUC Activity in RP | Cell Death in NBL | LUC Activity in RP | Cell Death in NBL | |
GFP | 1.00 ± 0.19 | 0/8 | 1.00 ± 0.34 | 0/8 | ND | 0/8 |
NlMLP | 0.74 ± 0.17 | 1/8 | 0.14 ± 0.02* | 8/8 | ND | 0/8 |
BAX | 0.66 ± 0.21 | 3/8 | 0.09 ± 0.02* | 7/8 | ND | 0/8 |
INF1 | 0.92 ± 0.34 | 3/8 | 0.38 ± 0.11* | 7/8 | ND | 6/8 |
LaCl3 was applied to protoplasts immediately after transfection at a final concentration of 1 mm. For agroinfiltration of N. benthamiana leaves, LaCl3 was added to resuspended A. tumefaciens cultures at a final concentration of 1 mm.
Rice protoplasts and N. benthamiana leaves were incubated in the dark for 30 min before transfection and maintained in the dark after transfection.
N. benthamiana leaves were preinfiltrated with A. tumefaciens cells harboring the Bcl-xl expression vector.
LUC activity in rice protoplasts. Data represent means ± se of three repeats. Asterisks indicate significant differences compared with the corresponding GFP (*, P < 0.05, Student’s t test).
Number of cell death sites/total number of infiltrated leaves of N. benthamiana.