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. 2017 Nov 10;176(1):496–510. doi: 10.1104/pp.17.00905

Figure 3.

Figure 3.

BMVCP5 displays greater insert stability than BMVF13m in N. benthamiana and maize. A, N. benthamiana leaves were harvested at various dpai with BMVCP5:PDS or BMVF13m:PDS. Virion RNA was isolated and subjected to RT-PCR assays. RT-PCR products were obtained using pooled cDNA reactions from three plants representing leaves infiltrated with BMVCP5:PDS or BMVF13m:PDS, respectively. The experiment was repeated twice with similar results. B, RT-PCR products from extracts of individual inoculated (top) or systemically infected (bottom) maize leaves infected with BMVF13m:PDS or BMVCP5:PDS. Each lane represents extract from an individual plant. All samples were taken at 5 dpi. C, RT-PCR products from extracts of inoculated (top) or systemically infected (bottom) maize leaves infected with BMVF13m:HSP70-1 (F13m:HSP70-1) or BMVCP5:HSP70-1 (CP5:HSP70-1). Each lane represents extract from an individual plant. Samples were taken at 5 dpi (inoculated leaves) or 10 dpi (systemically infected leaves). All PCR products were visualized on 1% agarose gels after electrophoresis. Lanes labeled 1 and 2 are PCR products from pC13/F3CP5 or pC13/F3CP5:PDS plasmid DNA and show the positions of bands expected when the full-length insert is absent (410 bp; lane 1) or present (660 bp; lane 2). Lane L is 1 kb plus DNA ladder.