Figure 4.

Increased stomatal density is not associated with altered stomatal anatomy. A, Box plot showing stomata number measured per mm² of leaf area of the wild type (WT) and three ZjPCK_pro:ZmSHR1 T2 lines. B, Box plot of stomatal complex sizes calculated from stomatal height multiplied by stomatal length. Error bars = 50 µm. All P values were determined by one-way ANOVA where transgenics were individually compared with the wild type: ****, P = 0.0001; ***, P < 0.001 and **, P < 0.01. C to F, Representative examples of stomatal morphology in the wild type (C), 207_₂₉ (D), 207_₃₀ (E), and 207_₃₁ (F) lines photographed by DIC microscopy. G, Morphology of the smallest complexes in line 207_₃₀. The white arrow corresponds to the data point indicated by the black arrow in B, and the yellow arrow corresponds to the green arrow in B. H and I, Positive imprints of the abaxial epidermis of wild-type (H) and ZjPCK_pro:ZmSHR1 line 207_{_31} (I) leaves visualized by scanning electron microscopy. Papillae cells are indicated as P, arrows point to stomata, and veins are indicated. J and K, Representative examples of stomatal morphology in wild-type (J) and 207_₃₁ (K) lines photographed by scanning electron microscopy. Bars = 30 µm (C–G), 10 µm (H and I), and 5 µm (J and K).