Table I. Quantification of leaf anatomy traits.
Measurements were taken from the sixth fully expanded adult leaf on the first tiller for four individuals from each line. For distance and cell number between veins, measurements were taken from three or four pairs of intermediate veins for each leaf. Bundle sheath cell size measurements were taken from six cells in each leaf. Values represent means ± se. Significance was tested using a one-way ANOVA (P < 0.05). There were no significant differences between the three transgenic lines and the wild type (WT) for any of the measurements.
| Line | Leaf Width | Leaf Length | Total No. of Veins | Average Distances between Veins (n = 3) | Average Cell No. between Veins (n = 3–4) | Average Bundle Sheath Cell Size (n = 6) | Vein No. per mm of Leaf Area |
|---|---|---|---|---|---|---|---|
| mm | µm | µm | |||||
| WT1a | 11 | 465 | 52 | 212 ± 6.7 | 6.5 ± 0.6 | 9.9 ± 1.7 | 4.7 |
| WT1b | 11 | 435 | 54 | 185 ± 2.9 | 6.3 ± 0.6 | 10.2 ± 3.1 | 4.7 |
| WT1c | 12 | 550 | 55 | 219 ± 4.2 | 6.7 ± 0.6 | 9.8 ± 2.8 | 4.6 |
| WT1d | 13 | 565 | 57 | 213 ± 43.2 | 6 ± 0 | 10 ± 2.4 | 4.4 |
| WT total | 11.9 ± 0.4 | 503.8 ± 31.8 | 54.5 ± 1.0 | 207.3 ± 7.6 | 6.4 ± 0.1 | 10.0 ± 0.1 | 4.6 ± 0.1 |
| 207_29-1a | 10 | 455 | 44 | 150 ± 8.4 | 8.4 ± 0.5 | 8.6 ± 0.2 | 4.4 |
| 207_29-1b | 11 | 478 | 55 | 203 ± 6.3 | 5.2 ± 0.5 | 11.2 ± 3.8 | 5.0 |
| 207_29-1c | 10 | 490 | 49 | 194 ± 8.3 | 6.8 ± 0.5 | 8.3 ± 1.6 | 4.9 |
| 207_29-1d | 9 | 405 | 45 | 199 ± 16.2 | 6.25 ± 0.5 | 9.2 ± 0.9 | 5.0 |
| 207_29 total | 10.0 ± 0.4 | 457.0 ± 18.8 | 48.4 ± 2.5 | 186.4 ± 12.4 | 6.7 ± 0.7 | 9.3 ± 0.7 | 4.8 ± 0.1 |
| 207_30-1a | 10 | 512 | 44 | 215 ± 13.3 | 7.4 ± 1.1 | 8.7 ± 0.3 | 4.4 |
| 207_30-1b | 15 | 595 | 55 | 202 ± 4.2 | 7.3 ± 0.6 | 9.0 ± 1.7 | 3.7 |
| 207_30-1c | 9.5 | 435 | 51 | 194 ± 3.5 | 7.3 ± 0.6 | 10.4 ± 1.7 | 5.4 |
| 207_30-1d | 10 | 443 | 51 | 205 ± 8.1 | 7.6 ± 0.6 | 8 ± 2.2 | 5.1 |
| 207_30 total | 11.1 ± 1.3 | 496.3 ± 37.2 | 50.3 ± 2.3 | 204.1 ± 4.4 | 7.4 ± 0.1 | 9.0 ± 0.5 | 4.6 ± 0.4 |
| 207_31-1a | 10 | 420 | 44 | 187 ± 16.6 | 8 ± 1.0 | 9.4 ± 0.5 | 4.4 |
| 207_31-1b | 9.5 | 579 | 45 | 219 ± 2.5 | 8.6 ± 0.5 | 8.6 ± 1.7 | 4.7 |
| 207_31-1c | 11 | 560 | 52 | 211 ± 5.1 | 7.5 ± 0.5 | 10.1 ± 2.2 | 4.7 |
| 207_31-1d | 10 | 513 | 51 | 180 ± 10.4 | 6.5 ± 0.5 | 9.0 ± 1.9 | 5.1 |
| 207_31 total | 10.1 ± 0.3 | 518 ± 35.5 | 48.0 ± 2.0 | 199.1 ± 9.3 | 7.7 ± 0.4 | 9.2 ± 0.3 | 4.7 ± 0.1 |