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. 2017 Nov 8;176(1):836–850. doi: 10.1104/pp.17.01290

Figure 5.

Figure 5.

SUSIWM1 regulates expression of ClTST2. A, Association of sugar content with ClTST2 promoter haplotype in cultivated, semiwild, and wild watermelons. B, XP-CLR estimation of the ClTST2 genome region. ClTST2 is localized in the 40-kb regions surrounded by red frame, which resides in the genome region that went through selective sweep during watermelon domestication. C, Y1H assays showing that SUSIWM1 activated AUR1-C expression when both the 97103 (97) and PI296341-FR (PI) sequence motifs were used in the presence of 200 to 600 ng/mL AbA. No activation was detected for the empty vector. D, EMSA of GST-SUSIWM1 fusion protein binding to the SURE cis-elements in ClTST2 from 97103 (97) and PI296341-FR (PI). FP assay showing probed SUSIWM1 dose-dependent binding activity to SURE cis-elements in ClTST2 from 97 and PI. E, LUC assay of strawberry fruits cotransformed with p35S::SUSIWM1::tNOS as the effector or with p35S::MYC::tNOS as the control, with the −1,500 bp promoter region of the sweet and unsweet lines fused to LUC. Relative fluorescence results are presented as histograms, representing the means of five replicates. F, Digital expression (in RPKM) of SUSIWM1 in high-sugar-content fruit line 97103, in low-sugar fruit (PI296341-FR) and low-sugar tissue (97103 rind).