Effects of hws and HWS overexpression on miRNA and miRNA target steady-state levels. A, Phenotype of 28-d-old T1 35S::HWS plant in the hws-1 background. Note hyponastic, serrated leaves. B and C, Levels of mature miRNAs and miRNA targets in Col-0, hws-1, and 35S::HWS as measured by RT-qPCR. Dots represent biological replicates (n = 3 to 4); bars indicate mean of biological replicates as means of technical replicates. D, Mature miRNA levels in Col-0, hws-1, hws-5, MIM156, hws-5; MIM156 and 35S::HWS as determined by RNA blotting.