Epistasis analysis of hws-1 and mutations in major miRNA biogenesis factors. A, Rosette phenotype of single and double homozygous F3 plants between hws-1, hyl1-2, se-3, ago1-25, ago1-27, hst-3, and abh1-753 at approximately 21 DAS. B, Abscission phenotype of double mutants of hws-1, hws-5, hyl1-2, hst-3, se-3, and ago1-27 as well as T1 of 35S::HWS and 35S::mHWS in hws-1 background. C, Odds ratio of enrichment of differentially expressed genes also found in the miRNA-mutants hyl1-2, se-3, cpl1-7, and ago1-27 as calculated by two-tailed Fisher’s exact test in 35S::HWS, hws-1, hws-5, hws-5; MIM156 (compared to Col-0 and to MIM156; n = 2), MIM156 and miRNA-mutants cpl1-7, hyl1-2, and se-3 (n = 3; data from Manavella et al., 2012) and ago1-27 (n = 3, data from SRA; PRJNA309714), with 95% confidence intervals. For P values, see Supplemental Table S2.