Figure 2.

The rapid germination of etr1;etr2;ein4 triple mutant plants under various inhibitory conditions is differentially rescued by truncated and etr1 receiver domain mutants. A, Germination time-courses were determined for etr1-6;etr2-3;ein4-4 triple mutant plants and these triple mutants were transformed with a cDNA for full-length ETR1 (cETR1) or a truncated ETR1 lacking the receiver domain (cetr1-ΔR) or genomic DNA for full-length wild-type ETR1 (gETR1), a D659A mutant, or an E666A mutant. The times for 50% germination were then calculated. Conditions used were control (using standard conditions described in the “Materials and Methods”), 150 mm NaCl, 150 mm KCl, 100 mm ethanol, 100 μM CuSO₄, 100 μM NaSO₄, 300 μM ZnSO₄, and 300 μM NaSO₄. B, Percent seed germination of seeds kept in darkness 7 d after planting. For both panels, data are the average ± sd. Data were analyzed using two-way ANOVA and Tukey’s multiple comparisons test. NR, never reached 50% germination in the time-course of the experiment. (*) Denotes the etr1-6;etr2-3;ein4-4 triple mutant transformed with the indicated transgene is statistically different from the triple mutant. (^#) The transformant is statistically slower than triple mutant transformed with full-length ETR1 transgene (P < 0.05).