Figure 5.

The D659A and E666A mutant transgenes oppositely affect the transcript abundance of genes encoding for ABA signaling proteins. The transcript levels of selected genes encoding for ABA signaling proteins were determined for etr1-6;etr2-3;ein4-4 triple mutant plants and these triple mutants transformed with genomic DNA for full-length wild-type ETR1 (gETR1), a D659A mutant, or an E666A mutant. For comparison, data for wild-type seeds are shown. The transcript levels of genes in germinating seeds encoding for proteins in the ABA signal transduction pathway were analyzed with real-time qRT-PCR as described in Figure 3. The average ± se for two biological replicates with three technical replicates each is shown. Data were analyzed using a two-way ANOVA and Tukey’s multiple comparisons test. In each panel, different letters indicate significant difference (P < 0.05).