Table I. Relative activities of recombinant TcADH1, TcADH2, and TcALDH1 with selected substrates.
The activities of TcADH1 and TcADH2 were measured with 0.3 mm alcohols and 1 mm NAD+. The activities of TcALDH1 were measured with 0.04 mm aldehydes and 1 mm NADP+. Data are expressed as relative mean percentages from triplicate independent assays.
| Alcohols | TcADH1 | TcADH2 | Aldehydes | TcALDH1 |
|---|---|---|---|---|
| Cis-3-hexenol | N.D.a | N.D. | Trans-2-heptenal | 47 |
| Perillyl alcohol | N.D. | 35 | Octanal | 90 |
| Nerol | 100b | 42 | Dodecanal | 47 |
| Geraniol | 48 | 38 | Citral | 20 |
| (S)-β-Citronellol | 8 | 22 | Perillaldehyde | 40 |
| Trans,trans-farnesol | N.D. | N.D. | Farnesal | 54 |
| Trans-chrysanthemol | N.D. | 100c | Trans-chrysanthemal | 100d |
| 8-Hydroxygeraniol | N.D. | 70 | (S)-(−)-Citronellal | 84 |
| Benzyl alcohol | N.D. | N.D. | Benzaldehyde | 90 |
| Cinnamyl alcohol | N.D. | N.D. | Trans-cinnamaldehyde | 8 |
a
N.D., Not detectable (less than 5% of highest activity).
b
100% relative activity, corresponds to 0.44 μmol min−1 mg−1 citral.
c
100% relative activity, corresponds to 0.5 μmol min−1 mg−1 trans-chrysanthemal.
d
100% relative activity, corresponds to 0.11 μmol min−1 mg−1 trans-chrysanthemic acid.