Table II. Kinetic properties of recombinant TcADH2 and TcALDH1.
Data are presented as means ± sd from triplicate independent assays. All assays were performed by GC-MS.
| Enzyme | Substrate | Km | Kcat | Kcat/Km |
|---|---|---|---|---|
| μm | s−1 | m−1 s−1 | ||
| TcADH2 | Trans-chrysanthemola | 236.0 ± 5.8 | 0.75 ± 0.0032 | 3,186.2 |
| NAD+b | 192.6 ± 8.7 | 0.64 ± 0.0034 | 3,345.5 | |
| TcALDH1 | Trans-chrysanthemalc | 4.4 ± 2.2 | 0.11 ± 0.0049 | 25,122.4 |
| NADP+d | 20.4 ± 7.1 | 0.090 ± 0.0013 | 4,391.8 | |
| Trans-chrysanthemale | 4. 6 ± 1.8 | 0.096 ± 0.0032 | 20,992.3 | |
| NAD+f | 68.6 ± 17.1 | 0.086 ± 0.0012 | 1,256.07 |
a
Kinetic parameters were determined with 1 mm NAD+.
b
Kinetic parameters were determined with 0.64 mm trans-chrysanthemol.
c
Kinetic parameters were determined with 1 mm NADP+.
d
Kinetic parameters were determined with 0.04 mm trans-chrysanthemal.
e
Kinetic parameters were determined with 1 mm NAD+.
f
Kinetic parameters were determined with 0.04 mm trans-chrysanthemal.