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. 2017 Nov 22;176(1):80–93. doi: 10.1104/pp.17.00962

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Figure 5. — Photoactivation localization microscopy of EB1c protein in root epidermal cells of Arabidopsis expressing an EB1c-DRONPA marker and STED of actin filaments in Arabidopsis cotyledon epidermal cells expressing a GFP-FABD2 actin marker. A to C, Wide-field (WF) epifluorescence (A) and PALM (B) imaging and quantitative assessment (C) of the precision of localization of B at approximately 40 nm. D and E, Comparison of SIM (D) and PALM (E) localization of EB1c. F and G, Comparison of CLSM (F) and STED (G) imaging of actin filaments in Arabidopsis cotyledon epidermal cells. H and I, Volumetric CLSM (H) and STED (I) imaging of actin microfilament organization in Arabidopsis cotyledon epidermal cells. Bars = 2 μm (A, B, D, and E) and 10 μm (F–I).