Table 2. Summary of final conditions used for the phenotypic assay.
Condition | Value |
---|---|
Cell number (cells / well) | 25,000 |
Compound pre-incubation time (h) | 1.5 |
Stimuli | 50 μg/mL poly I:C + 50 ng/mL IL-4 |
Stimulation time (h) (in the presence of test compounds) | 24 |
Assay buffer | ITS / BSA in BEBM medium without supplements |
Final DMSO concentration (%) | 0.1 |