Skip to main content
. 2018 Jan 10;13(1):e0189247. doi: 10.1371/journal.pone.0189247

Table 2. Summary of final conditions used for the phenotypic assay.

Condition Value
Cell number (cells / well) 25,000
Compound pre-incubation time (h) 1.5
Stimuli 50 μg/mL poly I:C + 50 ng/mL IL-4
Stimulation time (h) (in the presence of test compounds) 24
Assay buffer ITS / BSA in BEBM medium without supplements
Final DMSO concentration (%) 0.1