Fig 5. Analysis of the mRNA expression of other 8 genes using RT-qPCR in PM-resistant and PM-susceptible plants.
Total RNA was extracted from pumpkin leaves that were sprayed with a spore suspension or water. The pumpkin β-actin gene was used as an internal reference gene. The expression levels of the genes of plants sprayed with water only at each time point were used as controls. The relative gene expression in A, B, C, D, E and G (Y-axis) was transformed to a log10 scale. The values are the means ± SEs of three biological replicates.*indicates significant differences (P<0.05).