Fig 1. Fibrosis was more severe in diaphragm than in quadriceps muscles of mdx^5cv mice.

(A) H&E staining of quadriceps (Qua) and diaphragm (Dia) muscles in the mdx^5cv mice at 3 months and 6 months of age (arrows: representative areas of myonecrosis and inflammation; asterisks: representative areas of endomysial fibrosis and inflammation). (B) Collagen III immunostaining of quadriceps (Qua) and diaphragm (Dia) muscles of mdx^5cv mice at 3 months and 6 months. (C) Hydroxyproline assay of quadriceps (Qua) and diaphragm (Dia) muscles from the ColI-GFP/mdx^5cv mice at 3 months and 6 months. Fold change refers to the comparison to the mdx^5cv quadriceps at 3 months. We used 5 mice/group/time point for tissue collections for HE staining, collagen III immunostaining, and protein preparation for hydroxyproline assay. *p<0.05, ***p<0.001. Bar = 50μm.