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. 2017 Jun 6;16(6):987–996. doi: 10.1177/1533034617712397

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© The Author(s) 2017

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — Electrosensitization efficiency at different applied voltages when using a pulsed electric field (PEF)–delivering probe with 6.5 mm gap between the electrodes. KLN 205 cells seeded in 1% agarose were exposed to either a single train of 300 pulses (300 ns, 20 Hz) or 2 trains of 150 pulses each with a 2-minute interval. The pulse amplitude was varied from 4.4 to 6.4 kV. A, Each sample at 4.4 kV, a representative propidium (Pr) fluorescence image. The arrows identify the nanosecond pulsed electric field (nsPEF) delivering electrodes. Scale bar: 1 mm. The quantification in (B) shows the Pr uptake (left Y-axis) and the percentage of cell death (right Y-axis) measured within the region of interest (white square) shown in (A). Mean ± standard error (SE), n = 5 to 6. *P < .01 and **P < .001.