Figure 2.

Isolation and identification of CIK cells. Cytokine-induced killer cells were isolated from human PBMCs, cultured in media supplemented with differentiation factors, and then observed under a light microscope. A, Cytokine-induced killer cells demonstrated sufficient cell numbers (×200). Cytokine-induced killer cells were identified by flow cytometry with CD3 and CD56 double-positive staining at a rate of 42.5% (11.7%). CIK indicates cytokine-induced killer; PBMC, peripheral blood mononuclear cells.