Video 8. The myosin VI mechanochemical cycle.
Morph between indicated states of the myosin VI mechanochemical cycle, aligned in the reference from of the actin filament. To generate this morph, the full ADP and rigor density maps were aligned, and then the LPF ADP MDFF and LPF rigor MDFF models were rigid body fit into their corresponding densities. The grafted lever arm models were then superimposed on the LPF MDFF models using overlapping Cα coordinates. For the Pre-Powerstroke (2V26) and PiR (4PFO) states, the X-ray structures were superimposed on the LPF ADP MDFF model based on the Cα coordinates of the full motor domain. For the post-rigor (2VAS) state, the x-ray structure was superimposed on the LPF rigor MDFF model based on the Cα coordinates of the full motor domain. The lever arms of crystal structures were extended in a manner analogous to the procedure described in the Experimental Methods after superposing 3GN4 utilizing overlapping Cα coordinates. Myosin, magenta, actin density map, grey. Nucleotides and ions are displayed in ball and stick representation and colored by heteroatom.