Table 1. Data collection and refinement statistics, related to Figure 1 and Figure 1—figure supplement 1.
| Data collection | |||||
|---|---|---|---|---|---|
| EM | Tecnai F20 | ||||
| Voltage (kV) | 200 | ||||
| Detector | Gatan K2 Summit | ||||
| Pixel size (Å/pixel) | 1.27 | ||||
| Electron dose (e-/Å2) | 36 | ||||
| Defocus range (μm) | −1.5 – −3.0 | ||||
| Conditions | Myosin VI (Rigor) | Myosin VI (ADP) | Actin | ||
| Number of micrographs | 778 | 377 | 442 | ||
| Reconstruction and Refinement | |||||
| Software | EMAN2/SPARX and FREALIGN | ||||
| Segments | 56,116 | 36,114 | 63,139 | ||
| Asymmetric Units | 168,348 | 108,342 | 189,417 | ||
| Rise (Å) | 28.06 | 28.06 | 28.11 | ||
| Twist (°) | −166.73 | −166.69 | −166.65 | ||
| Maps | HR Rigor | LPF Rigor | HR ADP | LPF ADP | HR Actin |
| Resolution (Å) | 4.6 | 7.5 | 5.5 | 7.5 | 5.5 |
| Map sharpening B- factor (Å2) | −150 | −150 | −200 | −200 | −350 |
| Model Building | |||||
| Software | Direx, Coot, MDFF, Phenix | ||||
| Validation | |||||
| Molprobity score | 1.44 | 1.78 | 1.40 | 1.42 | 1.63 |
| Clash score | 0.41 | 2.81 | 0.33 | 0.39 | 1 |
| Ramachandran statistics (%) | |||||
| Favored | 90.26 | 93.3 | 91.31 | 91.01 | 90.87 |
| Outlier | 1.36 | 1.12 | 1.29 | 1.52 | 0.76 |
| Structure Deposition | |||||
| PDB Accession Code | 6BNP | 6BNV | 6BNQ | 6BNW | 6BNO |
| EMDB Accession Code | EMD-7116 | EMD-7117 | EMD-7115 | ||