Table 2.

Overview of the observed peptides (blue) and impurities (purple).

¹Purity assigned based on UV chromatogram with internal normalization to the most abundant peak.

²The peptide C₁VFSLFKKC₉N (disulfide bond between C₁ and C₉) was the most abundant peak and was not the desired sequence.

³Structural deviations are defined as follows: (a) Chemical modifications of an amino acid are depicted in between brackets following the modified amino acid; (b) X signals a “semi-identified” impurity with already part of the peptide sequence allocated in the MS² fragmentation pattern.

⁴Solely observed in UV spectrum, no signal in TIC.