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. 2017 Nov 18;8(67):110743–110755. doi: 10.18632/oncotarget.22500

Figure 3. Spindle poisons induce mitotic aberrations in ADF cells.

Figure 3

(A) ADF cells were incubated for 48 h in the absence or presence of 8.5 µM SI113, 0.625 nM VCR, 3.5 nM EPO-A or the combination of SI113 and VCR or SI113 and EPO-A. Cells were then stained with Hoechst 33342 to highlight nuclei (blue, left column) and α-tubulin (fluorescent antibody, red, central column). Merging is shown in the right column. Cells incubated in the presence of VCR or EPO-A, regardless the presence or absence of SI113, appear larger, with microtubule destabilization and hyperconsensed chromatin, harboring multiple nuclei, micronuclei and/or multi-lobulated nuclei. (B) ADF cells stained with Hoechst 33342 and γ-tubulin (merged). VCR- or SI113 plus VCR- cells, treated as above, showed condensed chromatin (M-arrested), displaying large γ-tubulin foci (arrows), suggestive of overduplicated, clustered centrosomes. In both panels, control values were generated by adding the same amount of solvent(s) to the cells.