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. 2017 Nov 28;8(67):110785–110796. doi: 10.18632/oncotarget.22720

Figure 6. Knockdown of miR-19a/b expression suppresses U87 tumor growth in vivo.

Figure 6

(A) U87 cells pretreated with a lentivirus containing luciferase reporter or cotransfection of miR-19a/b inhibitors were implanted in the right forebrain of nude mice, and tumorigenicity was analysized by bioluminescence imaging. Tumor formation assessed by bioluminescent signal were examined at day 4, 10, 23 after tumor implantation. (B) Tumor growth curves were analyzed. The data are described as mean ± SD. *p < 0.05. (C) Overall survival was determined by Kaplan-Meier analysis, and log-rank test was used to evaluate the statistical of the differences. (D) At the end of experiment, Immunohistochemistry was used to assess the expression of RUNX3, β-catenin and CyclinD1 in tumor sections between control and AS-miR-19a/b group. (E) TUNEL analysis on intracranical tumor sections indicating more apoptotic cells compared with control group. *p < 0.05.