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. 2017 Nov 10;8(67):111012–111025. doi: 10.18632/oncotarget.22451

Figure 1. Mist1 marks long-lived, multipotent isthmus progenitors in the antrum.

Figure 1

(A) Longitudinal stomach section of Mist1-CreERT; R26-TdTomato mice stained with GS-II (green). Areas indicated by white boxes in the corpus and antrum are enlarged in right. Macroscopic cut line of the section is shown by blue line in S1D. (B) In situ hybridization of Mist1 in the antrum. (C) Relative gene expression per Gapdh in each part of the stomach (n = 3). (D–F) Lineage tracing in Mist1-CreERT; R26-mTmG mice from days 1-540. Arrows indicate Mist1+ cells and their progeny. Quantification of the Mist1-traced cell position is shown in (E). A total of 50 glands were analyzed at each time point. (G) Lineage tracing frequency in Mist1-CreERT; R26-mTmG, Mist1-CreERT; R26-TdTomato, and Mist1-CreERT; R26-LacZ mice at day 1 and 360. (H) Immunofluorescence of the indicated markers (green) in Mist1-CreERT; R26-TdTomato mice 12 months after TAM induction. (I) Antral gland culture of TAM-induced Mist1-CreERT; R26-mTmG mice. The arrow indicates Mist1+ cells.