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. 2017 Nov 10;8(67):111012–111025. doi: 10.18632/oncotarget.22451

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Copyright: © 2017 Sakitani et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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(A) Alcian blue (blue) staining in Mist1-CreERT; LSL-Kras^G12D mice at the indicated time points. (B) GSII (green) and CD44 (red) staining in Mist1-CreERT; LSL-Kras^G12D mice at the indicated time points. (C) CD44 (green) and p-ERK (red) staining in Mist1-CreERT; LSL-Kras^G12D mice at day 30 after tamoxifen. (D-E) Immunofluorescence for β-catenin (green) and Ki67 (red) in Mist1-CreERT; Apc^flox/flox mice on days 7, 30, and 60 after TAM induction (D). The arrows indicate the nuclear β-catenin⁺ cells. Ki67⁺ cell ratio in total nuclear β-catenin⁺ cells is quantified (E). A total of 300 cells from three mice are analyzed at each time point. (F) Longitudinal H&E stained section of Mist1-CreERT; Apc^flox/flox mouse stomach 60 days after TAM induction. (G) Gross picture Mist1-CreERT; Apc^flox/flox mice 60 and 90 days after TAM induction. (H) H&E staining of Mist1-CreERT; LSL-Trp53^R172H; Apc^flox/flox mice and Mist1-CreERT; LSL-Kras^G12D; Apc^flox/flox mice 150 days after TAM induction.