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. 2017 Nov 10;8(67):111012–111025. doi: 10.18632/oncotarget.22451

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Copyright: © 2017 Sakitani et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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(A) Longitudinal stomach section of Mist1-CreERT; Apc^flox/flox; Cxcr4-EGFP; Cxcl12-dsRED mice 60 days after tamoxifen stained with DAPI. Arrows indicate dysplastic lesions. (B) Relative gene expression per Gapdh in normal antrum and antral tumors of Mist1-CreERT; Apc^flox/flox mice 60 days after tamoxifen. (C–D) Cxcl12-dsRED; Cxcr4-EGFP mouse antrum without (control antrum) and after MNU treatment (MNU-treated), and Mist1-CreERT; Cxcl12-dsRED; Cxcr4-EGFP; Apc^flox/flox mice 6 weeks after TAM induction (Apc^flox/flox). Cxcl12⁺ and Cxcr4⁺ areas were measured in (D). A total of 30 high power fields (HPF) from three mice were analyzed. (E) 3D reconstructed images of Mist1-CreERT; Apc^flox/flox; Cxcr4-EGFP; Cxcl12-dsRED mouse antrum 60 days after tamoxifen stained with DAPI. (F) GFP and Ki67 staining of Cxcr4-EGFP; Cxcl12^flox/flox (control) mice and Tie2-Cre; Cxcr4-EGFP; Cxcl12^flox/flox mice 40 weeks after the start of 5 cycles of MNU treatment. Cxcr4⁺ and Ki67⁺ epithelial cell ratio of Cxcr4-EGFP; Cxcl12^flox/flox (control) mice and Tie2-Cre; Cxcr4-EGFP; Cxcl12^flox/flox mice were quantified. The total 1500 cells from three mice are analyzed. (G) Macroscopic antral tumor size was measured in Cxcl12^flox/flox (control, N = 13) mice and Tie2-Cre; Cxcl12^flox/flox mice (N = 7) 40 weeks after the start of 5 cycles of MNU treatment. (H) Relative mRNA expression/Gapdh of the indicated genes from the MNU-induced tumor tissues in Cxcl12^flox/flox mice (Ctr) and Tie2-Cre; Cxcl12^flox/flox mice (Tie2).