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. 2017 Nov 27;8(67):111144–111160. doi: 10.18632/oncotarget.22676

Figure 4. Cepharanthine hydrochloride (CEH) dose-dependent reduction of p-gp mediated drug resistance in esophageal cancer cell lines.

Figure 4

(A) Relative expression of MDR1 mRNA was examined by qRT-PCR in Eca109 cells and Eca109/CDDP cells. Expression of the housekeeping gene GAPDH was used as reference. (B and C) Relative expression of MDR1 mRNA was examined by qRT-PCR in Eca109 cells and Eca109/CDDP cells in the presence or absence of CEH under cisplatin (cDDP) conditions. Expression of the housekeeping gene GAPDH was used as reference. (D and E) Western blotting detection of P-gp protein expression in Eca109 and Eca109/CDDP cells treated with cDDP and cDDP combined with various concentrations of CEH for 48 h. GAPDH was used as the internal loading control. (F and G) Eca109 and Eca109/CDDP cells were treated for 48 h with cDDP and cDDP combined with various concentrations of CEH, western blotting detection of JNK, p-JNK, Jun and p-c-Jun proteins. GAPDH was used as an internal control. Data are presented as mean ± SD of three independent experiments. ***, P < 0.01 versus control.