Figure 8. CFI-400945 induced polyploidy in multiple RT and MB cell lines, while sparing non-neoplastic human fibroblasts.

(A) Flow Cytometry Cell Cycle analysis of MON rhabdoid cells exposed to 100nM CFI-400945 for 3-72 hours revealed a time-dependent induction of polyploidy compared to the control (0.1% DMSO). (B) Flow Cytometry Cell Cycle analysis of G401 (RTK), BT-16 (AT/RT) and two MB cell lines (DAOY and D283) treated with 100nM CFI-400945 for 48 hours also displayed extreme polyploidy. No effect was observed in the non-neoplastic human fibroblast cell line HFF-SCC058 (that do not overexpress PLK4), when treated with 100nM of CFI-400945.