Figure 3. The effects of leucine treatment on zebrafish sperm motility were reduced after inhibiting the PI3K/Akt signaling pathway.

(A) Representative immunoblots showed p-Akt and Akt proteins after LY294002 and Leucine treatment. Actin was used as the control. Relative protein level was shown from 3 independent experiments. (B) Quantification of the ratio of pAkt/Akt from 3 independent experiments. (C-E) Comparisons of semen kinematic parameters. Six kinematics parameters including motile (C), progressive and slow sperm (D), VAP, VSL and VCL (E) were assessed with the computer-assisted sperm analysis (CASA) system. Bars represent mean ± SEM of more than three separate experiments. ^*P < 0.05, ^**P < 0.01 and ^***P < 0.001.