Figure 4. The imBMSCs and imBMSC41 cells are positive for most of the mesenchymal stem cell markers.
Subconfluent imBMSCs (A) and imBMSC41 (B) cells were stained for MSC markers as described in the Methods. Antibodies against CD73/NT5E, CD90/Thy-1, CD105/Endoglin, CD117/c-Kit, CD29/integrin β1, and BMPRII were from Santa Cruz Biotechnology. Minus primary antibodies and isotype IgG were used as negative controls (data not shown). Cell nuclei were counterstained with DAPI. Representative images are shown.