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. 2017 Dec 5;8(67):111882–111901. doi: 10.18632/oncotarget.22948

Figure 4. ICAM-1depletion attenuates miR-124 expression and promotes MCP-1 production in macrophages.

Figure 4

Downregulation of miR-124 (A) and upregulation of MCP-1 (B) in RAW264.7 cells transfected with siRNA against ICAM-1. Twenty-four h after transfection, expressions were measured with real-time qPCR. The data in graph were shown in mean ± SEM of 3 different transfections. (C) MCP-1 protein levels were upregulated when ICAM-1 was downregulated. RAW264.7 cells were co-transfected with siRNA-ICAM-1 and miR-124 mimics, MCP-1 protein levels were measured with Western blot analysis 48h later. (D) MCP-1 protein expression was determined by ELISA. (** p <0.01).