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. 2017 Dec 5;8(67):111882–111901. doi: 10.18632/oncotarget.22948

Figure 7. In primary macrophages confirm ICAM-1 regulation of MCP-1 expression via miR124.

Figure 7

(A) Real-time qPCR analysis of expression levels of miR-124 in porcine alveolar macrophages (primary macrophages) after transfected with mimic-NC, miR-124 mimics, inhibitor-NC and miR-124 inhibitor. (B) PAM cells were transfected with miR-124 mimic or mimic control, inhibitor-NC or miR-124 inhibitor for 48h, MCP-1 protein levels were measured with Western blotting. (C) In PAM cells transfected with siRNA-ICAM-1, knockdown of ICAM-1 was measured with Western blot. (D) Real-time qPCR and Western blot analysis of the expression of Sp1 after transfection with ICAM-1 siRNA and negative control. (E) The mRNA expressions of M1 and M2 markers in PAM cells transfected with siRNA-ICAM-1 or miR-124 mimics for 24h. (* p < 0.05, ** p <0.01, *** p <0.001).