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. 2017 Dec 5;8(67):111943–111965. doi: 10.18632/oncotarget.22955

Figure 1. CAV1 expression is suppressed by DNA methylation in cancer cell lines.

Figure 1

Colon cancer cells (A) HT29(US), (B) DLD-1 and (C) HT29(ATCC) and breast cancer cells (D) MCF-7 were treated either with 5-aza dideoxycytidine (Deoxy, 1, 2.5, 5 or 10 μM) for 72 h, Trichostatin (TSA, 50 ng/ml) for 24 h or the combination of 2.5 μM Deoxy and 25 ng/ml TSA. Cells were harvested and total protein extracts were separated by SDS-PAGE (50 μg total protein per lane) and analyzed by Western blotting with antibodies against caveolin-1 (CAV1) and β-actin. The graphs show the expression of CAV1 normalized to β-actin (mean ± SEM) of 3 independent experiments. Significant differences in comparison with the untreated condition (Basal) are indicated *** p ≤ 0.001, ** p ≤ 0.01, * p ≤ 0.05.